##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840879.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2840906
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.91815920695722	37.0	34.0	39.0	26.0	39.0
2	35.74963268760036	37.0	35.0	39.0	31.0	39.0
3	36.071225869493745	37.0	35.0	39.0	32.0	39.0
4	36.184645320894106	37.0	35.0	39.0	32.0	39.0
5	37.00972999458624	38.0	35.0	40.0	33.0	41.0
6	37.03299686790059	38.0	35.0	40.0	33.0	41.0
7	37.03609376727002	38.0	35.0	40.0	33.0	41.0
8	36.96811862131306	38.0	35.0	40.0	33.0	41.0
9	36.88406832186634	38.0	35.0	40.0	33.0	41.0
10	36.81486047056819	38.0	35.0	40.0	32.0	41.0
11	36.73360012615694	38.0	35.0	40.0	32.0	41.0
12	36.821998686334574	38.0	35.0	40.0	33.0	41.0
13	36.936469562878884	38.0	35.0	40.0	33.0	41.0
14	36.97965472986434	38.0	35.0	40.0	33.0	41.0
15	37.00828573701488	38.0	35.0	40.0	33.0	41.0
16	37.00747120812868	38.0	35.0	41.0	33.0	41.0
17	37.011149260130395	38.0	35.0	41.0	33.0	41.0
18	37.04679070690829	38.0	35.0	41.0	33.0	41.0
19	36.986664817491324	38.0	35.0	41.0	33.0	41.0
20	37.04261492636504	38.0	35.0	41.0	33.0	41.0
21	36.956955985168115	38.0	35.0	41.0	33.0	41.0
22	36.89100554541403	38.0	35.0	41.0	33.0	41.0
23	36.862908170844086	38.0	35.0	41.0	33.0	41.0
24	36.794252608146834	38.0	35.0	41.0	32.0	41.0
25	36.68766738163189	38.0	35.0	41.0	32.0	41.0
26	36.64679613722647	38.0	35.0	40.0	32.0	41.0
27	36.612643567833906	38.0	35.0	40.0	32.0	41.0
28	36.65663527665277	38.0	35.0	40.0	32.0	41.0
29	36.605400878755184	38.0	35.0	40.0	32.0	41.0
30	36.58677173603632	37.0	35.0	40.0	32.0	41.0
31	36.46085357631573	37.0	35.0	40.0	31.0	41.0
32	36.37491138074487	37.0	35.0	40.0	31.0	41.0
33	36.358457582002245	37.0	35.0	40.0	31.0	41.0
34	36.36201602732488	37.0	35.0	40.0	31.0	41.0
35	36.28615392355812	37.0	34.0	40.0	31.0	41.0
36	36.246382267845156	36.0	34.0	40.0	31.0	41.0
37	36.20234559978586	36.0	34.0	40.0	31.0	41.0
38	36.139840313885166	36.0	34.0	40.0	31.0	41.0
39	36.03435961161876	36.0	34.0	40.0	31.0	41.0
40	35.95612013168869	36.0	34.0	40.0	31.0	41.0
41	35.876697340399424	36.0	34.0	40.0	31.0	41.0
42	35.80692679708057	35.0	34.0	40.0	31.0	41.0
43	35.713828980342925	35.0	34.0	39.0	31.0	41.0
44	35.63323976131968	35.0	34.0	39.0	31.0	41.0
45	35.451590048390706	35.0	34.0	39.0	30.0	41.0
46	35.34016013273168	35.0	34.0	39.0	30.0	41.0
47	35.24216160657677	35.0	34.0	39.0	30.0	41.0
48	35.13706770986508	35.0	34.0	39.0	30.0	40.0
49	35.23175878012582	35.0	34.0	39.0	30.0	40.0
50	35.214671409768776	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	9.0
14	661.0
15	3367.0
16	304.0
17	2.0
18	0.0
19	1.0
20	16.0
21	65.0
22	246.0
23	663.0
24	1577.0
25	3520.0
26	7301.0
27	13834.0
28	24786.0
29	42752.0
30	70242.0
31	110698.0
32	164252.0
33	251755.0
34	440962.0
35	169731.0
36	191292.0
37	248953.0
38	310824.0
39	438436.0
40	344657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.16649406914555	13.142180698692602	35.69132523216185
2	26.94168691255536	26.05911635231859	30.053792698526454	16.945404036599594
3	27.196781590098368	18.268714276361134	26.927325296929922	27.607178836610576
4	21.73602364879373	20.537286344567544	31.22077956820817	26.505910438430558
5	20.064268229923833	24.774526154684455	29.744384361890187	25.416821253501524
6	20.226505206437665	30.329338598320394	27.95847521882104	21.485680976420902
7	27.854881506111077	26.984454959086996	23.072674703070074	22.08798883173185
8	24.9589391553258	25.44308048207157	25.157854571745773	24.44012579085686
9	23.718208205410527	27.04725182740999	27.358490566037734	21.87604940114175
10	25.64840230546171	22.724194323923424	25.340190770127556	26.287212600487308
11	24.86833425674767	25.563253412819716	26.765017920339496	22.803394410093116
12	25.750588016639764	23.39039024874459	27.3978442088545	23.461177525761148
13	22.52489170708218	22.158072107982456	29.405971193696658	25.911064991238707
14	20.859331494952666	25.15876977274151	27.894129548812952	26.08776918349287
15	20.07250503888548	28.98504913573346	27.743121384516066	23.19932444086499
16	23.938384444962278	31.31522127096074	22.455266031329444	22.291128252747537
17	24.747703725501655	29.47816647224512	22.03620957539602	23.737920226857206
18	23.28950693898355	26.557196894230223	24.50239465860539	25.65090150818084
19	22.64256543511119	25.795045665009685	26.648329793382814	24.914059106496307
20	25.40045323569312	21.872247797005603	27.10494469017982	25.622354277121453
21	25.160388974503206	25.896668175574977	24.710532485059346	24.232410364862478
22	25.029902432533845	27.21754961269398	24.965767962755546	22.78677999201663
23	24.887201477275205	27.34546655186761	23.58715846282841	24.180173508028776
24	25.69254315348695	27.23036242663432	24.203159133037136	22.873935286841593
25	24.722918476237744	27.51713257582472	24.653891833442646	23.106057114494885
26	24.403985836354288	27.432052226034426	25.31364423351813	22.850317704093158
27	25.311653058077095	27.61369939738268	24.52324999937089	22.551397545169337
28	25.652822658424512	25.803843596882302	25.410096218396617	23.133237526296572
29	25.177133998685914	25.541878985127774	25.936870583493587	23.344116432692726
30	26.015013543295996	25.94850759627772	25.314078278196618	22.72240058222967
31	23.782516250261803	27.870410869080946	25.296974783323677	23.050098097333574
32	24.64116893505854	25.388460943196034	26.948309008489918	23.0220611132555
33	23.06000191609387	27.773968549761147	27.11081221716482	22.055217316980162
34	24.913966301133843	24.955412363720047	27.038237872390035	23.092383462756075
35	23.759485102081577	25.76330582393272	25.464135374914047	25.013073699071654
36	24.708150225639336	26.39767249349217	26.406153876855686	22.488023404012807
37	23.593086905713758	26.10142191204407	27.152334788163767	23.153156394078405
38	23.589438019917118	27.259381598217324	25.867973690138168	23.28320669172739
39	22.810721094291868	27.722552960185503	26.499023234221912	22.967702711300714
40	22.86731260150871	29.511796287081975	24.959060049614827	22.661831061794484
41	23.474523483741166	26.52266332755133	26.09485912708151	23.90795406162599
42	24.139582042471694	26.920802117951897	25.978104304302697	22.961511535273715
43	23.069942358946708	26.58216489336649	26.120216271700663	24.227676475986144
44	23.521693947011723	27.79858900558196	25.411089451049197	23.26862759635712
45	23.98760456270617	27.750475609518894	25.92488825193793	22.337031575837006
46	22.56379961202797	29.520565450378545	25.689865382923365	22.22576955467012
47	23.523837492053605	27.15974357646831	24.545517044118732	24.77090188735935
48	23.031821496128902	28.26407004000745	25.090795502599008	23.613312961264647
49	23.66612731272118	27.11686060610119	26.096041990211276	23.120970090966356
50	24.66048530134156	29.576370651930716	25.92979842232691	19.83334562440081
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20511.0
1	16321.0
2	10769.0
3	8258.0
4	6876.0
5	6327.0
6	5795.5
7	5320.5
8	5046.5
9	4786.5
10	4419.5
11	5299.0
12	6476.0
13	7857.0
14	9540.0
15	9197.5
16	8644.0
17	11461.5
18	13928.5
19	14372.0
20	14596.5
21	17692.5
22	21329.0
23	22112.0
24	22764.0
25	28628.5
26	35105.0
27	41544.5
28	48847.5
29	55181.0
30	61551.0
31	71420.5
32	80461.0
33	86922.5
34	93155.0
35	98760.5
36	107580.0
37	119169.5
38	129178.5
39	134589.0
40	138114.5
41	153036.0
42	178797.5
43	190035.5
44	191814.5
45	186639.0
46	181126.0
47	179870.0
48	179350.5
49	180422.0
50	180860.0
51	177595.0
52	176074.5
53	179891.0
54	179199.5
55	176953.5
56	176315.0
57	167551.5
58	150999.0
59	142324.0
60	145088.5
61	133380.0
62	110285.5
63	93403.0
64	79740.5
65	71505.0
66	63675.5
67	61086.0
68	63372.0
69	57758.5
70	46472.5
71	40479.5
72	36574.0
73	31456.0
74	27899.0
75	25289.0
76	23249.0
77	18197.5
78	12291.0
79	11443.0
80	11043.0
81	8249.5
82	5860.0
83	5448.0
84	4990.5
85	3961.5
86	3012.0
87	1880.5
88	858.0
89	625.5
90	620.0
91	503.5
92	253.5
93	70.5
94	45.5
95	37.0
96	24.5
97	24.0
98	192.5
99	361.0
100	361.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	20256.0
25	18829.0
26	20122.0
27	18506.0
28	19084.0
29	18037.0
30	23654.0
31	21003.0
32	19750.0
33	19677.0
34	18400.0
35	17881.0
36	20526.0
37	19147.0
38	19876.0
39	19897.0
40	20208.0
41	20633.0
42	22592.0
43	21571.0
44	24884.0
45	29603.0
46	30356.0
47	57842.0
48	207799.0
49	490695.0
50	1600078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.96801806945982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.47274896071322	6.825375989059755
2	13.72201783062531	2.735626433702459
3	5.60557752051369	1.6762949404271472
4	2.8854124417244678	1.150473734278147
5	1.6703308628067424	0.8324944111217012
6	1.0949701222702262	0.6548809178584946
7	0.7219353905569071	0.5037385512637648
8	0.5265807327311788	0.41991730071150263
9	0.4158788937661254	0.3730939494990929
>10	2.9862037286128738	5.960630529001829
>50	0.4860749756850541	3.437839594755282
>100	0.7087855876566596	17.831828607056195
>500	0.6172267511463759	44.64183678642912
>1k	0.08413514706348078	11.067959858422856
>5k	0.0017675451063756467	1.396898576878819
>10k+	3.5350902127512934E-4	0.49110981953383026
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	13937	0.4905829337542319	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	9506	0.3346115640573817	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	9022	0.31757474552132314	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	8229	0.2896611151512933	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	6527	0.22975064996870717	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	6358	0.22380184349640575	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	4329	0.15238096579049076	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	4050	0.14256015510544875	No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG	3698	0.1301697416246789	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	3517	0.12379853469280575	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	3495	0.12302413385025762	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	3424	0.12052493113112507	No Hit
CTTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAA	3231	0.11373132373968023	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3017	0.1061985155439849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04076164434866905	0.0	0.0	0.0	0.0
2	0.07170247801229608	0.0	0.0	0.0	0.0
3	0.10299531205889952	0.0	0.0	0.0	0.0
4	0.13728014936080252	0.0	0.0	0.0	0.0
5	0.1813857973477475	0.0	0.0	0.0	0.0
6	0.24492186647499073	0.0	0.0	0.0	0.0
7	0.30011552652569284	0.0	0.0	0.0	0.0
8	0.3551683864232044	0.0	0.0	0.0	0.0
9	0.4151140516440882	0.0	0.0	0.0	0.0
10	0.4780517201202715	0.0	0.0	0.0	0.0
11	0.5529574016176529	0.0	0.0	0.0	0.0
12	0.6218438765661377	0.0	0.0	0.0	0.0
13	0.6913991522422777	0.0	0.0	0.0	0.0
14	0.7692264369183633	0.0	0.0	0.0	0.0
15	0.8529321279901553	0.0	0.0	0.0	0.0
16	0.9332234153470759	0.0	0.0	0.0	0.0
17	1.0136203028188895	0.0	0.0	0.0	0.0
18	1.0860267815971383	0.0	0.0	0.0	0.0
19	1.1669516696434166	0.0	0.0	0.0	0.0
20	1.2446733542046093	0.0	0.0	0.0	0.0
21	1.322113438459421	0.0	0.0	0.0	0.0
22	1.393147115744062	0.0	0.0	0.0	0.0
23	1.452564780390481	0.0	0.0	0.0	0.0
24	1.5156784490581525	0.0	0.0	0.0	0.0
25	1.5794609184534794	0.0	0.0	0.0	0.0
26	1.641659386125412	0.0	0.0	0.0	0.0
27	1.700126649737795	0.0	0.0	0.0	0.0
28	1.7595091143459165	0.0	0.0	0.0	0.0
29	1.8283251892178058	0.0	0.0	0.0	0.0
30	1.8937972604514193	0.0	0.0	0.0	0.0
31	1.9576501299233413	0.0	0.0	0.0	0.0
32	2.0177717953357135	0.0	0.0	0.0	0.0
33	2.0833846667225173	0.0	0.0	0.0	0.0
34	2.1513559406752636	0.0	0.0	0.0	0.0
35	2.2192568145514144	0.0	0.0	0.0	0.0
36	2.2883896897679823	0.0	0.0	0.0	0.0
37	2.352664959699476	0.0	0.0	0.0	0.0
38	2.4162010288267193	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGA	80	0.0	73.11981	44
TGCACTA	95	0.0	73.11981	44
CGGCTAT	65	0.0	73.119804	44
TTACGCG	85	0.0	68.81865	44
TAATCGT	85	0.0	68.81865	44
TCACTCG	105	0.0	66.15602	44
ATAGCGA	95	0.0	61.57458	44
CCCGTCA	125	0.0	61.420647	44
TACGCTT	90	0.0	60.933178	44
GCTAGTT	75	0.0	58.495853	44
CGATCTA	30	4.8627044E-7	55.88731	43
ATGTCGC	90	0.0	55.887306	43
GAGACAC	240	0.0	54.83986	44
ATGCACC	160	0.0	52.554867	44
CACGACG	80	0.0	52.394356	43
TAGCCGT	260	0.0	52.027554	44
AGAACGG	100	0.0	51.18387	44
CCGTACC	20	3.8312442E-4	50.902477	42
TGTCGTA	115	0.0	50.902477	42
AGGTCGA	210	0.0	50.56471	43
>>END_MODULE