FastQCFastQC Report
Sun 19 Mar 2023
SRR7458460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259800122
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC23863710.9185411390992342No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7434310.2861549849464659No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT5969890.22978780587331674No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4709370.1812689679953268No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3724640.14336559857350645No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3236520.12457730870503594No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2710180.1043178878876739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2938000.0141.56191
TATCAGC4692750.0139.626951
GATAGAC2509100.0139.273241
AGTGGTC6118750.0139.239211
TAGCGAC3008600.0138.282352
AGATCGC2926750.0137.258761
CCGAAGT3206800.0137.134381
AGGCTAA3570450.0137.074171
CGAACTT3250400.0136.930511
GTACGCA1828350.0136.688281
ACTTCGA3344100.0136.376052
GGCTAAC3591700.0136.126132
AACCGAG2794850.0135.811131
ACGCTTA2798600.0135.516882
CTCAATG4041050.0134.717851
ATCAGCA4853650.0134.645342
CTGGCAT3960250.0134.587951
GTCGTAG2994200.0134.53121
AGAGTCA2526150.0134.515271
ACGTATC1984000.0134.501621
Produced by FastQC (version 0.11.5)