FastQCFastQC Report
Sun 19 Mar 2023
SRR7458460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259800122
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3960945315.246125634998739No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC30446151.1719066860176455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28000401.0777670073611436No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG15217910.5857545363277389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14935730.5748931095575083No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8733960.3361799807007019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA7330100.2821438243974343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5842790.22489558338236654No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5786860.2227427745395747No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC4691980.18059960726269406No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4115420.1584071619489078No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3458520.1331223393343903No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3448840.13274974520604726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3263250.12560617658216494No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3102580.11942180689199214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2984260.11486753651332005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2922120.11247569775968004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT2756440.10609848751341232No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2712340.10440102872622976No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2609820.10045491818514235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT302348200.066.143161
AGCAGTG311618600.064.179352
AGTGGTA312723400.063.9588555
GTATCAA315775000.063.3694539
CAGTGGT316586200.063.1997264
GGTATCA317809700.062.9426428
GCAGTGG318408400.062.814383
GTGGTAT319813750.062.549476
TGGTATC320246400.062.478887
GTGACTG1928900.031.4532591
TGACTGG1923550.031.3489672
GACTGGA1981600.030.273643
AGTTCAG2023200.029.7342959
TGGAGTT2074800.028.9358716
ACTGGAG2567200.023.5422234
ATGGGAT17636000.022.90324225-29
CGATCGT340200.021.364576120-124
AAACCGC1525800.021.26811885-89
CTGGAGT2867600.021.144385
CGACCTA413450.020.81091745-49
Produced by FastQC (version 0.11.5)