FastQCFastQC Report
Sun 19 Mar 2023
SRR7458459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203609310
Sequences flagged as poor quality0
Sequence length150
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25707561.2625925602321426No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7145160.35092501418525507No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6035440.2964225948214254No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3910510.19205948883182208No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3888760.19099126655848891No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2590030.12720587285522453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGA3403850.0141.494962
ACGTATC3766850.0140.9351
CGCTCGA2084150.0139.373442
GTCACTA2286950.0139.307742
GATAGAC1858450.0139.1651
ACGTGAT7990300.0139.119872
TATCAGC2305700.0139.117951
GCTGATC2992450.0138.510712
AGTCACT2301700.0138.418411
ACGCTTA1946250.0138.301672
AGATCGC3134850.0138.242571
ACCTTAC6066950.0138.190482
ACGTGAC2977050.0138.148765
CTACGTG3174100.0138.049293
CAATGGA9408500.0137.807341
CACTTCG3509050.0137.742521
ATCAGCA2323150.0137.734992
GATCGCA3154650.0137.291662
CCGAAGT2526300.0136.959371
CGAACTT2292900.0136.70511
Produced by FastQC (version 0.11.5)