FastQCFastQC Report
Sun 19 Mar 2023
SRR7458459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203609310
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3048403414.971827172342955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20597071.0115976523863275No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG12923820.6347362014045429No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC12669360.6222387375115607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10471150.51427658195001No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6503610.3194161406470068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4176810.20513845855083934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA3746570.1840077941426156No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2967740.14575659629709467No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2679310.13159074111100322No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2653180.13030740097297122No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2564490.12595150978115885No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2330180.11444368629312676No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2125100.10437145531311902No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2055490.10095265290177546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT215882800.068.931121
AGCAGTG223579850.066.535452
AGTGGTA224011300.066.427955
GTATCAA224704950.066.257179
GGTATCA228373850.065.14968
CAGTGGT230788700.064.478384
GCAGTGG232077050.064.1040653
GTGGTAT232850800.063.898416
TGGTATC232954800.063.8600237
TGACTGG1474650.022.5474832
AGTTCAG1484400.022.3828569
CGATCGT281400.021.95136120-124
GACTGGA1525300.021.7036843
ATGGGAT13827100.021.19290525-29
CGACCTA393200.020.85709445-49
AAACCGC1062700.020.71931885-89
TACGCAC404300.020.47653850-54
GTGACTG1641250.020.2543741
GAAACCG1101350.020.09943285-89
TGGAGTT1691850.019.6213116
Produced by FastQC (version 0.11.5)