FastQCFastQC Report
Sun 19 Mar 2023
SRR7458458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102741888
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1467790.1428618870620715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC7155000.0141.09912
CCTAATC3768700.0140.972991
CGGATTG6345500.0140.681921
GTACGCA2068500.0140.670761
ATAGCGA1658100.0140.403141
CGACACA4962250.0139.432141
TCACGCA4901500.0138.906172
TAGCGAC1675150.0138.829822
GGATTGC6431400.0138.386782
CTAATCC3844800.0137.837362
TACGCAA2128950.0137.211492
GCCAAGA6057400.0137.180921
GTCGTAG1918200.0137.030471
TATCAGC2112700.0136.550571
AGCACCT3271550.0136.30481
CCAAGAC6119300.0135.907842
GGCTAAC2595100.0135.766432
GATTGCG2461350.0135.387653
CACGCAT1835050.0135.048173
AGGCTAA2612400.0134.828021
Produced by FastQC (version 0.11.5)