FastQCFastQC Report
Sun 19 Mar 2023
SRR7458458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102741888
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG67499776.5698393628896525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC48401854.711014265184614No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA13403251.3045555479766928No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC4727130.4600976380733825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT4137540.40271208564903926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3511700.34179827413722436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2924090.28460543765752094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2324400.22623683925294424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1867390.18175546861665615No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGCTCTCTAAAAAAAAAAAAAA1812710.17643339394347124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1722560.1676589785852485No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG1545580.15043328773557285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1493430.1453574612138722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC1377580.1340816318267385No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1273920.12399227080584697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCGTGGAGAACAAAAAGTT1052860.10247621690580574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT74277100.091.033431
AGCAGTG75954950.089.0532462
AGTGGTA76130100.088.952555
GTGGTAT76187450.088.862986
CAGTGGT76266750.088.705724
TGGTATC76074900.088.62857
GGTATCA76067000.088.497668
GTATCAA76160600.088.338859
GCAGTGG77319850.087.48583
GTATCGA273650.042.907849
TGGTATG608400.038.70457
GGTATGA609700.038.5517358
GGTATCG289000.035.190138
GTATGAA816650.028.8964619
CATGGGG21269050.025.01082625-29
ATGGGGG6967000.024.50684425-29
GGGGCGT7227900.024.06169125-29
ATGGGGC11292600.024.05291625-29
TACGCAC403650.023.69335650-54
CGATAGG296050.023.44998735-39
Produced by FastQC (version 0.11.5)