FastQCFastQC Report
Sun 19 Mar 2023
SRR7458457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110175710
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5577490.5062359026322589No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3070890.278726590461727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC4991600.0141.627911
GAGCTGA4828900.0140.841861
ACTTCGA2751750.0139.15352
CAAGCTA5085800.0139.118422
GTCACTA3322750.0138.154722
ACGTATC1466900.0138.054021
ACAAGCT5148300.0137.538861
AGCTGAA4955050.0137.285632
AACATCG3139400.0137.10522
CTCAATG3189650.0137.096761
AGTGGTC3608950.0136.913561
GCATACA5159000.0136.518982
CACTTCG2810400.0136.375461
CGACTGG3610400.0135.184571
AGAGTCA2494150.0134.610431
CTGGCAT2234700.0133.788971
GTGGTCA3689900.0133.493422
GACTAGT1580050.0133.289441
AAGCTAG1606300.0133.287023
CTGTAGC2085000.0132.775591
Produced by FastQC (version 0.11.5)