FastQCFastQC Report
Sun 19 Mar 2023
SRR7458457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110175710
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1607695614.592105646516822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC48377934.39097964515046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14724051.3364152588624116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA12795111.1613367411020088No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC5476050.4970287915548718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT4364080.3961018268001177No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG3779950.34308378861366085No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3355140.3045262880538732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2212330.20080015821999242No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGCTCTCTAAAAAAAAAAAAAA2050170.18608185052767076No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1722010.15629670096975096No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1429950.12978813569706063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1409610.12794199374798673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1401700.12722404965668022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1357720.12323224420337296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1179560.10706171078906593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCGTGGAGAACAAAAAGTT1176660.10679849487695608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT129274350.063.4732971
AGCAGTG131870800.062.2394452
AGTGGTA132460050.062.044765
CAGTGGT132872200.061.7899554
GTGGTAT133170850.061.6985056
GTATCAA132625500.061.5604449
TGGTATC133034650.061.5067637
GGTATCA132899200.061.4683888
GCAGTGG133992850.061.258383
GTATGAA923850.030.2512729
GGTATGA932950.029.725418
TGGTATG975400.028.3945397
GTATCGA568500.026.0319869
TACGCAC435900.023.2377650-54
CATGGGG20302350.022.70969625-29
ATGGGGG5765350.022.66273325-29
CGATAGG258850.022.27682335-39
ATGGGGC11423750.022.15161525-29
CGACCTA454300.022.0689845-49
GGTATCG596350.021.7473358
Produced by FastQC (version 0.11.5)