FastQCFastQC Report
Sun 19 Mar 2023
SRR7458456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117878797
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1294370.10980515859862398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA2240950.0140.23611
TCACGCA5861950.0139.583022
GTCGTAG3619500.0138.409941
ATCCGTC4052800.0137.665392
AGCCATG3772700.0137.477161
ATAGCGA1350450.0137.409291
TCGTAGA3657000.0136.801732
GCCATGC3776950.0136.689012
TAGCGAC1355100.0136.646762
TACGCAA2309200.0136.513232
CTAACGA4985250.0136.457532
ATGAATC2681150.0135.733582
CACGCAT2163300.0135.647283
CGACACA3690050.0135.601151
TTCACGC6058900.0135.584081
GCGAGTA2215350.0135.441771
CCTAATC2195200.0135.255661
AACTCAC4104500.0135.054371
GATAGAC1464300.0134.917281
GTCTGTC4422050.0134.734181
Produced by FastQC (version 0.11.5)