FastQCFastQC Report
Sun 19 Mar 2023
SRR7458456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117878797
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54023164.582941239212001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC34329742.9122913427764283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA11336580.9617149384379957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA3868490.32817521882243167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCAAGGCCAGTCCTGAGC3430010.29097768956702197No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC3155200.2676647607796676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT2999110.25442319368087885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2889630.24513568797279126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2703850.22937543212287786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCAAGGCCAGTCCTGAGCA2633150.2233777462116448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT2078700.1763421457380499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2077280.17622168302243532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1942780.16481165819837812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG1750550.14850423015429992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA1500690.12730788218003275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1436170.1218344635804181No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC1367620.11601916840057334No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGCTCTCTAAAAAAAAAAAAAA1362770.11560772884372073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC1315450.11159343609521226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1300470.11032263927837677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1278220.10843510729075391No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT1269440.10769027444350318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1180900.10017916962623906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT66103850.095.606831
AGCAGTG67670900.093.416382
GTGGTAT67824550.093.302136
AGTGGTA67874250.093.262785
TGGTATC67740150.093.025617
GGTATCA67640850.093.004638
CAGTGGT67993450.093.001274
GTATCAA67713100.092.858969
GCAGTGG69428500.091.067863
GTATCGA301050.039.4086849
TGGTATG638350.033.9211167
GGTATCG302750.033.782248
GGTATGA700700.031.0361798
GTATGAA837700.025.8833189
CATGGGG20528150.024.6841325-29
TACGCAC566150.024.57566850-54
CGATAGG500050.023.95091835-39
ATGGGGC9532100.023.95025625-29
CGACCTA586300.023.67711645-49
GGGGCGT5235500.023.55634925-29
Produced by FastQC (version 0.11.5)