FastQCFastQC Report
Sun 19 Mar 2023
SRR7458455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102738055
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8646500.841606355113497No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT5010460.48769270549262395No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3158940.30747516098100164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA5577550.0143.835541
ACGTATC1981650.0140.692931
TGCCTAA5716400.0140.353742
AGAGTCA3063100.0138.157461
ACTTCGA1780100.0137.787342
CATCAAG2542750.0137.559191
CGCTCGA2633250.0137.161872
ACGCTCG2642600.0137.014161
ATCCTGT3396800.0136.75151
GCCTAAT2486750.0136.357163
GCTCGAT1480900.0136.123023
AGTCACT1760250.0135.971041
CAAGCTA3305800.0135.267042
GTCACTA1771050.0134.98552
GCTGATC2381550.0134.539062
TCCTGTA3461950.0134.149062
CAAGACT1905200.0133.988721
CGAACTT1698100.0133.827761
CACTTCG1846900.0133.78021
AACCGAG2332850.0133.450531
Produced by FastQC (version 0.11.5)