FastQCFastQC Report
Sun 19 Mar 2023
SRR7458455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102738055
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1580798615.386689966050069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC44723304.353138669016072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA11324591.1022780215179273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9015580.8775307260780827No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC4400940.4283651272160058No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG3838440.373614236711022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT3743620.3643849399329197No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3625610.35289844644226526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2307100.22456138574941878No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1913250.1862260289042848No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1684950.16400446747799538No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGCTCTCTAAAAAAAAAAAAAA1512480.14721711443729396No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1396160.13589511695544557No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1335710.13001122125584333No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1257450.12239379069420771No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCAAGGCCAGTCCTGAGC1180150.11486980165236726No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1114220.10845251061060092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCGTGGAGAACAAAAAGTT1112320.10826757426933964No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1106640.10771471194388486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG1101550.10721927721913756No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1101300.10719494349002422No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1039950.10122344636561398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT117172750.056.6949351
AGCAGTG119486950.055.6222572
AGTGGTA119795650.055.552795
GTGGTAT120428400.055.248466
CAGTGGT120436350.055.2023624
TGGTATC120092500.055.190597
GTATCAA119885500.055.1584939
GGTATCA120016800.055.138878
GCAGTGG121530950.054.6939433
GTATGAA785950.028.636979
GGTATGA814450.027.5908188
TGGTATG877750.025.6090017
GGGGCGT7421800.021.55941825-29
ATGGGGC10002500.021.5533425-29
CATGGGG16702000.021.36149225-29
CGTGAGT9123500.021.1718670-74
GTGAGTC9216850.020.99388570-74
GTATCGA556300.020.9647779
CACGTTT9394350.020.92945760-64
TACGCAC261100.020.92105350-54
Produced by FastQC (version 0.11.5)