FastQCFastQC Report
Sun 19 Mar 2023
SRR7458454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86964829
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC3754550.0142.833911
ATAGCGA2063450.0142.78671
TAGCGAC2087400.0141.020682
AGTTAGC4282650.0139.444432
ATCAGCA3834300.0139.374532
AGAGATC4041950.0138.519152
GATGTAC3103850.0138.28462
ATCCGTC1711250.0137.382
GAGTTAG4349400.0137.277131
AGATTCG1359950.0136.900093
TCTATCG1253000.0136.112633
CAGATTC3753250.0135.882162
GTGCGAA2675100.0135.816962
CTCTATC3302100.0135.463042
ACCTTAC2668700.0134.94772
GTTAGCG1585350.0134.904133
GTCGTAG1580350.0134.763081
GGATTGC2487350.0134.274952
ATGAATC2691900.0134.241942
CCTAATC1352500.0134.237321
Produced by FastQC (version 0.11.5)