FastQCFastQC Report
Sun 19 Mar 2023
SRR7458454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86964829
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC4713570.5420087700051707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3324140.3822395833147674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2329830.26790485611142867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1526150.1754904847797723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA1517410.1744854807913208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC967420.11124267259813735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12874950.0125.977926
GGTATCA12885100.0125.887628
TGGTATC12950050.0125.181767
GTATCAA13067750.0124.2826169
AAGCAGT13139200.0123.649311
CAGTGGT13360500.0121.455694
AGCAGTG13465750.0120.563332
AGTGGTA13703800.0118.422525
GCAGTGG13980000.0116.081263
CAACGCA12814550.025.83013310-14
TCAACGC12855150.025.72403110-14
ATCAACG12861300.025.67971810-14
GTACATG12172550.025.67109320-24
AGAGTAC12898750.025.53419515-19
CAGAGTA12996250.025.37530515-19
TACATGG12110450.025.29742420-24
GAGTACA12843400.025.21578620-24
CGCAGAG13085150.025.1844315-19
AGTACAT13112400.025.10172320-24
GCAGAGT13209350.024.9522115-19
Produced by FastQC (version 0.11.5)