FastQCFastQC Report
Sun 19 Mar 2023
SRR7458453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179834604
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5461280.3036834890797769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC13345350.0141.826261
GTACGCA2394750.0141.514761
TAGCGAC1943100.0141.243642
ATAGCGA1946100.0140.963381
ATGCCTA2603350.0140.537321
GTCGTAG3611200.0140.459991
ATGAATC4386750.0140.416962
CATTCCG4678800.0140.30213
ACTTCGA1848800.0140.118352
GATAGAC1724200.0140.004031
AGATCGC2301950.0139.482511
CGCTCGA1503950.0139.469022
TCATTCC13592300.0139.35942
TACGCAA2447350.0139.162752
TCGTAGA3637400.0139.096712
ACGCTCG1511950.0139.038391
GCTGATC1738300.0139.035112
ACGTATC1598750.0138.948121
ACGCTTA1918300.0138.909322
TCACGCA2024000.0138.70842
Produced by FastQC (version 0.11.5)