FastQCFastQC Report
Sun 19 Mar 2023
SRR7458452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103099512
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3571200.0143.4541
GTACGCA2650350.0142.217211
TAGCGAC3613650.0141.478422
CCTAATC4080850.0140.52571
TATCAGC2511850.0139.908571
GATAGAC2149300.0139.681781
TACGCAA2721900.0138.827272
CTAATCC4151850.0137.958722
ACCTTAC4788300.0137.936632
TCACGCA3677000.0137.813062
ATCCGTC3174700.0137.369342
CCGACAA3487800.0136.863821
CGACAAC3490250.0136.666292
GCGAGTA1936400.0136.575651
GGCTAAC2038700.0134.951872
ATCAGCA2595450.0134.826342
CACCTTA4903950.0134.744281
CGGATTG3060950.0134.333471
AGCGACT1370550.0134.314623
GTGCGAA2491900.0134.224882
Produced by FastQC (version 0.11.5)