FastQCFastQC Report
Sun 19 Mar 2023
SRR7458452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103099512
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC20057201.9454214293468237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13839411.342335160616473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA5988640.5808601693478432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2042840.19814254794920852No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC1831220.17761674759430482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1729930.16779225880332005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT1719370.16676800565263586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1666290.1616195816717348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1437340.13941288102314198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC1340100.1299812165939253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT29308050.0116.790471
GTGGTAT29551800.0115.730446
GGTATCA29598100.0115.445588
TGGTATC29608950.0115.328157
GTATCAA29795900.0114.631379
AGTGGTA29989150.0114.070285
CAGTGGT30086800.0113.69754
AGCAGTG30128150.0113.550682
GCAGTGG30921500.0110.6099553
CATGGGG13908850.025.15146425-29
TACGCAC345950.024.3578750-54
GGGGCGT2786450.024.09924925-29
CGACCTA350400.024.04450845-49
AACGCGA450950.023.95832645-49
GTACATG27820150.023.67451320-24
ATGGGGC6095750.023.60581225-29
CAACGCA29537000.023.59012410-14
TACATGG27385100.023.5881420-24
AGAGTAC29520650.023.5773215-19
CAGAGTA29591850.023.53071215-19
Produced by FastQC (version 0.11.5)