FastQCFastQC Report
Sun 19 Mar 2023
SRR7458451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96567528
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4484630.46440352081913083No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1801900.18659481477044748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA2584050.0141.556181
CGCATAC2567100.0140.339311
AACATCG4606400.0139.907332
ACGTATC1268900.0138.44171
ACGCTCG2268700.0137.96931
ACTTCGA1576050.0137.911442
CGCTCGA2265550.0137.655532
CCGAAGT2231200.0137.642461
CTCAATG3110950.0137.131521
CAAGACT2865750.0136.46981
TGCCTAA2681750.0136.07832
ACGCTTA1476800.0135.760512
ACATCGA1532050.0135.704513
CGAAGTA2272600.0135.504822
AGTGGTC3147800.0135.345141
CATCAAG2487050.0135.030271
GAGCTGA2074650.0134.323171
CCGTGAG2582300.0134.316011
CGTGAGA2578400.0134.257662
ACCGAGA2152300.0134.182682
Produced by FastQC (version 0.11.5)