FastQCFastQC Report
Sun 19 Mar 2023
SRR7458451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96567528
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53211905.510330553351226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC21530622.2295921254192197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCTCTAAAAAAAAAAAAAA6203880.6424395579433285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2131720.22074915286223337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2005310.2076588312377635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1938720.20076313851587874No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGGCGTGGAGAACAAAAAGTTC1903570.19712319859735875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGAGAACAAAAAGTTCT1890990.19582048325809892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1511180.15648945678717177No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1269920.13150590330944373TruSeq Adapter, Index 7 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG1121440.1161301343449529No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1046080.10832626884681151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT48972650.082.61641
AGTGGTA49735000.081.194245
GTGGTAT49745000.081.15946
AGCAGTG49882750.080.960612
TGGTATC49801850.080.817457
GGTATCA49885450.080.731828
CAGTGGT50023250.080.716554
GTATCAA50029350.080.593559
GCAGTGG50914250.079.2906953
GGTATAA448850.027.4998938
CATGGGG13997400.024.69296325-29
TGGTATA502050.024.5439747
TACGCAC371350.024.2349150-54
CGACCTA371400.023.90234445-49
CGATAGG247300.023.73671735-39
ATGGGGC6489150.023.1831725-29
GGGGCGT3212650.022.85742625-29
ATGGGGG5274600.022.55461725-29
CGTGAGT3912450.022.22568970-74
TCGTGAG3949350.021.9487470-74
Produced by FastQC (version 0.11.5)