FastQCFastQC Report
Sun 19 Mar 2023
SRR7458450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197333084
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20818711.0550035289571615No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20405861.0340820498198873No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2956820.14983904067500411Illumina Single End PCR Primer 1 (100% over 50bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2724140.13804780956040802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC2306100.0141.319031
ATGAATC3205250.0141.028662
ACGTATC2910950.0141.017031
ATAGCGA1768750.0140.611041
ACGCTTA2315550.0140.251512
AGTTAGC2623100.0140.212422
GTGCGAA3371700.0139.822862
TGAGCCA3104950.0139.609592
ACGTGAT3919150.0139.289922
GGCTAAC1921300.0139.126852
AGAGTCA1507650.0139.057191
TGGCATA3200300.0139.049672
TAGCGAC1788800.0138.912432
TATCAGC2281400.0138.82821
ATAGACA2354900.0138.79822
AGTCACT1980400.0138.62811
ATCAGCA2293600.0138.502912
ACCGTGA4316350.0138.446784
CTGGCAT3213350.0138.303971
TACCGTG5162550.0138.231453
Produced by FastQC (version 0.11.5)