FastQCFastQC Report
Sun 19 Mar 2023
SRR7458450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197333084
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2041479210.345346855269337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13810250.6998446342631528No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4928820.24977159937357488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4916140.2491290309941135No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4687340.23753442174957343No Hit
CTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACC4682480.23728813765460638No Hit
GTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACC4625840.23441786375770624No Hit
CGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCG4430630.22452545260986242No Hit
CGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGT4257930.2157737523627817No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4248850.2153136166462589No Hit
GGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGG3804740.19280801388580132No Hit
CGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCC3518150.1782848536437002No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3309290.167700718648881No Hit
AGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCATAAGTACCACTGA3256800.16504074907175728No Hit
GACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGT3256660.16503365446819857No Hit
CCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCG3216770.16301219921135982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT3196460.16198297493794808No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAACATCTCGTATGC3183230.16131253490164882TruSeq Adapter, Index 22 (97% over 37bp)
GCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTC3170620.1606735138239668No Hit
CGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACA3094180.15679986028090453No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3078490.15600475792493063No Hit
CGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAGGACAGGCTGCGG3056100.15487012811293213No Hit
ATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCC3054150.15477131042050707No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3042190.1541652285736334No Hit
CACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGA3004230.15224157749442563No Hit
GAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCT2850840.1444684257810515No Hit
CTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGC2807810.14228784870153854No Hit
CAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAG2800780.1419315982514113No Hit
CATTGCCCTGGCCCATAAGTACCACTGAGTTCTCTTCCAGTTTGCAGGTG2800160.1419001792927941No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2760620.13989646054485216No Hit
GTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAGGACA2730560.13837314780931514No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2728460.1382667287559343No Hit
CGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGAC2712160.13744071419874024No Hit
GGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCT2636330.13359797285689815No Hit
CTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTAT2620070.13277398532929227No Hit
CTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCC2616210.13257837697403038No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2615250.1325297282639134No Hit
CCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTAC2595150.13151114589583973No Hit
AGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCAT2595030.13150506480707513No Hit
CTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAG2584160.13095421951648006No Hit
ACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGC2495740.12647347061175004No Hit
CCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATC2491140.12624036220910628No Hit
AAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGC2489220.1261430647888724No Hit
GGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGC2482880.1258217805991417No Hit
CTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCC2462620.12479509011271521No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2460920.12470894135521643No Hit
CTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTG2447790.12404356889288773No Hit
ATAAGTACCACTGAGTTCTCTTCCAGTTTGCAGGTGTTCCTGTGACCCTG2408070.12203072851179886No Hit
CCTGGCCCATAAGTACCACTGAGTTCTCTTCCAGTTTGCAGGTGTTCCTG2397310.12148545755257137No Hit
CGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTT2396400.12143934262943967No Hit
AGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTG2383840.12080285533874288No Hit
ATTGCCCTGGCCCATAAGTACCACTGAGTTCTCTTCCAGTTTGCAGGTGT2364150.11980505002394835No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2357710.11947869826024711No Hit
CTCCGGGACCCCCAGGACAGGCTGCGGCCCCTCCCCCGTCCTGGAGGTTC2352380.11920859656761865No Hit
ACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGA2340170.11858984578581866No Hit
CGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGG2335500.11835319008139558No Hit
AGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAGGACAGGCTGCGGCC2290270.1160611263745313No Hit
CCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCA2274190.11524626048007236No Hit
GGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGC2270580.11506332105973675No Hit
AGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCG2249930.11401686703482523No Hit
CGCCTCCGGGACCCCCAGGACAGGCTGCGGCCCCTCCCCCGTCCTGGAGG2244470.11374017749603507No Hit
CACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCG2221560.11257919629938992No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2210920.11204000642892704No Hit
CTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAGGACAGGC2209230.11195436442882534No Hit
CCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACC2205280.11175419525698996No Hit
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC2200320.11150284358805236No Hit
GCCATTGCCCTGGCCCATAAGTACCACTGAGTTCTCTTCCAGTTTGCAGG2190900.11102547812002977No Hit
CCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCT2172830.1101097675035576No Hit
CCATAAGTACCACTGAGTTCTCTTCCAGTTTGCAGGTGTTCCTGTGACCC2162290.10957564520706523No Hit
CCACTGCCTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGG2108700.10685993231626582No Hit
AAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGC2097930.10631415459964129No Hit
CTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCATAAGTACC2091250.10597564065841084No Hit
CCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGC2081840.10549878194778531No Hit
CCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACG2045700.10366736071484091No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2028320.10278661635876526No Hit
CCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAGGACAGG2027090.10272428519892793No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2020220.10237614286715349No Hit
CAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTG2019170.10232293334046308No Hit
ATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTC2017500.10223830485515546No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2008930.10180401376588225No Hit
GTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGC2000810.10139252675947638No Hit
TGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCG1986670.10067597180004545No Hit
GACAAGTTCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTG1983620.10052141079394472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT124600250.039.9822041
AGCAGTG127155150.039.1712882
AGTGGTA128074650.038.6090355
GTATCAA129357300.038.3813139
CAGTGGT128941300.038.354954
GGTATCA130721050.037.965668
TGGTATC131359500.037.7765277
GTGGTAT131353300.037.74786
GCAGTGG135598850.036.7714773
GGATCAA1815050.019.4795069
ACACTCG274450.018.5097430-34
TCCGACA257250.018.22993340-44
CTGGCGA352650.018.022545130-134
CTATAGG832400.017.82068830-34
CGGCTAT134600.017.555056140-144
GGACCAT756400.017.354193105-109
TGTGGTA824550.017.3227235
GGTCGGC389850.017.281612115-119
ACGTCAA379500.017.13624495-99
GGTAAGG359350.017.076283110-114
Produced by FastQC (version 0.11.5)