FastQCFastQC Report
Sun 19 Mar 2023
SRR7458449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7458449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192435579
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19509191.013803689597338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12501160.6496283101577593No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT5739810.2982717660542389No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3322890.1726754489615457No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2025020.10523106020846591Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA6846100.0141.372011
ATAGCGA2432400.0141.15971
ACGTGAT6845650.0140.883762
TAGCGAC2441950.0140.10342
ATGCCTA2831500.0139.861421
GTACGCA1648400.0138.288061
TGAGCCA3885100.0138.242192
TGCCTAA2869300.0137.880862
ACGTATC1717700.0137.760511
TACGCAA1669550.0137.567062
CAAGCTA1847650.0137.557662
GATAGAC1376850.0137.544711
AGAGTCA1952400.0137.454381
ACTTCGA1612250.0137.404722
GATCGCA2908750.0137.372442
TATCAGC2317550.0136.997331
ATCAGCA2319400.0136.782522
CGCATAC2049500.0136.771091
AACCGAG2048900.0136.765461
AGGCTAA1869450.0136.754781
Produced by FastQC (version 0.11.5)