FastQCFastQC Report
Sun 19 Mar 2023
SRR4896146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456068
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGATC456550.0144.756762
CTGATCG150450.0144.674673
CTGATCA136750.0144.650563
CGCTGAT457250.0144.535161
TGATCGA44500.0144.426994
CTGATCT150750.0144.002243
TGATCAG50750.0143.630514
TGATCGG44300.0143.44344
TGATCGT60500.0143.358524
GATCATG18200.0143.3235
TGATCTG59900.0142.980034
TGATCAA40100.0142.927864
TGATCAT45950.0142.550144
GATCGAG15150.0142.045875
GATCGTG19400.0141.927525
GATCAGT16250.0141.79425
GATCTGG19750.0141.613595
GATCGTA18300.0141.351975
GATCGGT15700.0141.223365
GATCTGA15000.0141.051065
Produced by FastQC (version 0.11.5)