FastQCFastQC Report
Sun 19 Mar 2023
SRR4896146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456068
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG92562.029521913398879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53931.1824991010112527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22280.4885236412113983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20380.446863187068595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13210.2896497890665427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCATGGTGGCTCAAG8490.1861564503538946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCATGGTGGCTCAA8450.18527938816141454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGACGCAGTGGCTCA7750.16993079979301332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGCCGGGCCCGGTGGCTC6040.13243639106449037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT5830.12783181455397002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5570.12213091030284957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5360.11752633379232921No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4790.10502819754948822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT146750.0102.222621
GGTATCA150000.0100.394488
GTATCAA150150.0100.138349
AGCAGTG150350.099.630312
GTGGTAT149900.099.494086
TGGTATC150600.099.127927
CAGTGGT150700.099.014034
AGTGGTA152350.098.036855
GCAGTGG156200.095.5740363
GCGCGTT259.385255E-486.99579145
AAATATA13350.049.96013145
CGCTGTT1600.049.84134145
ATTCACG700.001056287641.426567145
AGGGGTA2700.040.2802475
AGCGGTA800.002034843436.252225
GGGGTAT3100.035.0827946
TTCGATA900.003627037932.22066145
GCGGTAT950.00472321230.5281856
CGGTATC1000.00606922329.0017767
CCGCAAG3500.028.998596145
Produced by FastQC (version 0.11.5)