FastQCFastQC Report
Sun 19 Mar 2023
SRR4896145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627929
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT43000.6847907964116962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCG220850.0144.665993
GGATTGC629000.0144.642212
CGGATTG629350.0144.561771
GATTGCA172800.0144.22483
GATTGCT213900.0144.150473
ATTGCGA59150.0143.670784
ATTGCGT87750.0143.492074
ATTGCAG60450.0142.978044
ATTGCGG72000.0142.883164
ATTGCTA65000.0142.666444
ATTGCTG74300.0142.16544
ATTGCAT62550.0140.610124
TTGCGAG18150.0140.504845
TTGCGTA23450.0140.261415
TTGCGAT20250.0139.886875
ATTGCTT75200.0139.885914
TTGCTAG20800.0139.670995
TTGCTGA17250.0138.59585
GATTGCC23750.0137.879613
TTGCAGT20300.0137.4015
Produced by FastQC (version 0.11.5)