FastQCFastQC Report
Sun 19 Mar 2023
SRR4896145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627929
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG192173.0603778452659456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38670.6158339557497743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA30850.491297583006996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15130.2409508081327666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA14770.23521767588373846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11450.18234545625381213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7200.11466264498056308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCTGAGGCGGGTGGATCA7030.11195533252963312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT282850.099.515351
GTATCAA288650.097.726249
GGTATCA289350.097.638428
AGTGGTA287950.097.415785
GTGGTAT289300.096.92846
TGGTATC291750.096.189017
AGCAGTG292350.096.155732
CAGTGGT293000.095.8262944
GCAGTGG298550.094.142073
GGGTATC2900.052.5105557
CGCAAGG2950.044.239136145
AGGGGTA3600.042.303545
TTCGATA1053.8207436E-641.4303145
GGGGTAT4400.034.609236
TCGCACC900.003626469332.223568145
CAGGGGT4850.031.4055714
CGACCTA556.7775545E-926.36347845-49
CGGATTG1701.02984406E-425.5913411
AAACCGC3700.024.29753785-89
CGGTCTG1500.001493291924.167675145
Produced by FastQC (version 0.11.5)