FastQCFastQC Report
Sun 19 Mar 2023
SRR4896144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917315
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA922250.0144.139071
GACACAC923000.0144.021942
CACACGT95250.0141.721864
CACACTG106200.0141.641924
ACACACT324400.0141.611133
ACACACG286950.0140.876683
ACACTAG31650.0140.338155
ACACTGG33200.0139.024175
ACACGAT30500.0138.977665
CACTAGT10650.0138.793626
ACACTGA30450.0138.729955
ACACTGT34800.0138.04585
ACACGTG28950.0137.908815
ACACGGT33850.0137.852985
ACACTTG38050.0137.680565
CACACGA86000.0137.586784
CACACTA101950.0137.38324
ACACGTA27250.0136.93985
CACACTT119550.0136.916464
ACACATG34150.0136.641975
Produced by FastQC (version 0.11.5)