FastQCFastQC Report
Sun 19 Mar 2023
SRR4896144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917315
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG128261.398211083433717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG64270.7006317350092389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55010.5996849500989301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC34870.38013114360933814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18500.20167554220742057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT214700.0108.8238451
GGTATCA218050.0107.388728
GTGGTAT218250.0106.592756
GTATCAA219650.0106.375439
AGCAGTG220000.0106.151862
TGGTATC220850.0105.403527
CAGTGGT221400.0105.2186364
AGTGGTA222500.0104.627585
GCAGTGG224900.0103.581183
CGACACA2100.069.062341
CGATAAG1950.048.3331145
AGGGGTA2551.8189894E-1234.117485
GACACAC4300.033.7226032
AAATATA28500.033.57878145
CGGTCTG1352.1420814E-532.222065145
GGGTATC2859.094947E-1230.5245027
AATCGCG200.00607684428.999859140-144
GAGGTAT3301.8189894E-1228.558916
AGGTATC3409.094947E-1125.5867147
CATTCGG1751.2556567E-424.857021145
Produced by FastQC (version 0.11.5)