FastQCFastQC Report
Sun 19 Mar 2023
SRR4896143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438295
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5090.11613182901926784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC750.0144.912386
ATCGTGA3600.0144.912386
TCGAGAT1500.0144.912387
CTATCGA41900.0144.39364
CTCTATC439900.0144.368842
TCTATCG147000.0144.123733
CTATCGT53200.0143.959014
TATCGTG15150.0143.955865
CCTCTAT441700.0143.78051
TATCGAG12200.0143.724565
CTATCAG44250.0143.602434
CTATCGG50500.0143.47764
CTATCTG54350.0143.31264
TCTATCA127550.0142.924153
TATCGGG17650.0142.44935
TATCTAG13200.0142.167825
CTATCAT45550.0141.730994
TATCGGT16350.0141.367135
CTATCTA44550.0141.334294
TCTATCT152500.0141.206423
Produced by FastQC (version 0.11.5)