FastQCFastQC Report
Sun 19 Mar 2023
SRR4896143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438295
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG191254.363499469535358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG60291.3755575582655517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28970.6609703510192907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27460.6265186689330245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA26090.5952611825368759No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4740.10814633979397438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT276600.0101.78511
GTATCAA284650.099.101979
GGTATCA284450.099.0045248
CAGTGGT284350.098.8098454
AGCAGTG284500.098.808722
AGTGGTA283850.098.7545
GTGGTAT285300.098.201266
TGGTATC285450.098.1750647
GCAGTGG289300.097.0941243
AAATATA18250.056.410202145
GGGGTAT2450.050.3111956
AGGGGTA2650.046.514125
GGGTATC2550.045.4947787
CGAATGG700.001056060341.428017145
AGACGTA907.4761425E-540.27724145
TCTATCG750.001481742638.6705633
CTGACGT2051.1355951E-831.828842145
CGAAATA1050.007704308327.618677145
CGTGGGA2705.5460987E-926.851492145
AGGTATC2207.4632953E-726.3662917
Produced by FastQC (version 0.11.5)