FastQCFastQC Report
Sun 19 Mar 2023
SRR4896141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662577
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACAACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9260.13975734141088508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA664550.0144.45581
CGACAAC665100.0144.336332
GACAACT229350.0144.133763
GACAACG222150.0143.359133
ACAACGT80700.0142.468024
ACAACTG77350.0142.269384
GACAACA196900.0141.212543
CAACGTG24900.0140.23675
CAACATG27200.0139.831285
CAACTAG23900.0138.52635
CAACTGA21600.0138.51935
CAACTTG27400.0138.281835
CAACTGG25950.0138.191655
CAACGTT26750.0138.121225
ACAACGG80600.0137.970844
CAACTGT26700.0137.294545
AACTAGT7850.0136.585956
CAACGTA24650.0136.36885
CAACGGT24300.0136.246055
AACTAGG9150.0136.182516
Produced by FastQC (version 0.11.5)