FastQCFastQC Report
Sun 19 Mar 2023
SRR4896141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662577
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG142402.1491841702926604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG85611.2920762417047376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC39910.6023450859296353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36030.5437858543233466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21560.3253961426370067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCCTGGTGGCTCA16750.25280080654776727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACAGTGGCTCA12220.18443139438887857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC11870.17914898947594016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACGGTGGCTCAAG8870.13387123307932514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT204800.097.5121151
GGTATCA210050.095.178468
GTGGTAT208950.094.9854356
GTATCAA210850.094.864639
AGCAGTG210100.094.790462
CAGTGGT211500.094.025844
TGGTATC212450.093.5237
AGTGGTA212450.093.461795
GCAGTGG215350.092.3448643
AAATATA22500.059.609497145
CCGACGC1004.7009962E-850.74863145
ATCCGAC450.00963874148.332027145
GGTCTGA4600.037.825066145
AGCGGTA1402.7401378E-531.079975
GCCCCAA5950.030.461359145
AGGTATC2857.094059E-1127.9880357
GCGGTAT1606.8154564E-527.1929236
AAACCGC17450.025.50933885-89
GAAACCG17750.025.2415785-89
CGCTGTT2301.1024349E-625.21671145
Produced by FastQC (version 0.11.5)