FastQCFastQC Report
Sun 19 Mar 2023
SRR4896140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329825
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC154874.69552035170166No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG89082.7008261957098463No Hit
CCATCCTCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC27300.8277116652770409No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACC12880.3905101190025013No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGCACTCTGCGTTGATACC12550.38050481315849316No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG11240.34078678086864245No Hit
CCATCCTCTGCGTTCATACCACTGCTTCCCATGTACTCTGCGTTGATACC10360.31410596528462065No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATACC7470.2264837413780035No Hit
CCATCCTCTGGGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC6540.1982869703630713No Hit
CCATCCTCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC5810.1761540210717805No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG5590.16948381717577504No Hit
CCATCCTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATACC5430.16463275979686195No Hit
CCATCCTCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT4220.12794663836883197No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA4160.12612749185173955No Hit
CCATCCTCGTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4070.12339877207610096No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT3950.11976047904191617No Hit
CCATCCTCTGCGTTGATACCACGGCTTCCCATGTACTCTGCGTTGATACC3880.11763814143864171No Hit
CCATCCTCTGCGTTGATACCACTGCTTCGCATGTACGCTGCGTTGATACC3470.10520730690517699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATG202.1349042E-6144.912088
CGATATG100.00710159144.912088
CTGATGT151.2322377E-4144.912068
CCATCCT331600.0144.125441
CATCCTC331900.0143.99522
ATCCTCA98400.0143.807543
ATCCTCG89450.0143.697043
ATCCTCT135450.0142.718863
TCCTCGA24950.0141.71764
CCTCATG31100.0141.417425
CTCATGT25350.0140.910556
TCCTCTG75000.0140.564714
TCCTCAT50100.0140.428764
TCCTCAG25450.0140.356874
CTCTGTA2850.0139.827446
CCTCGTA9850.0139.762915
CTCAGGT2550.0139.229256
TCATGTA23500.0139.053927
CTCAGTG4200.0138.011496
CCTCGAG8150.0137.79985
Produced by FastQC (version 0.11.5)