FastQCFastQC Report
Sun 19 Mar 2023
SRR4896139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275062
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9800.3562833106717758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA100.007104344144.888938
TACGGAT3050.0144.888936
ACTTACG253.7212885E-8144.888937
CGTTCGA100.007104344144.888938
CTAGTAG253.7212885E-8144.888938
CGTAGAC202.1359629E-6144.888938
CGAGCTA151.2328012E-4144.888938
CGAGATG253.7212885E-8144.888938
CGATGTG306.530172E-10144.888938
ACGAGAT650.0144.888937
CGATAGG306.530172E-10144.888938
ACCTTAC275900.0144.442572
CACCTTA276050.0144.364071
CCTTACG85200.0144.123663
CTTACTG30800.0143.712884
CTTACGA23150.0143.637184
CTTACGG30250.0143.452014
CTTACGT30550.0143.228994
CCTTACT100150.0143.22523
CCTTACA82450.0142.868043
Produced by FastQC (version 0.11.5)