FastQCFastQC Report
Sun 19 Mar 2023
SRR4896139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275062
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG150375.4667674924198915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34191.2429924889661241No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27080.9845053115297643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21310.7747344235117901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15600.567144861885684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4330.15741905461314176No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3780.13742356268768496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTT256.456801E-6115.99883145
AAGCAGT238250.0109.7806551
AGTGGTA243850.0106.9027255
AGCAGTG245100.0106.742122
GTATCAA246250.0106.636499
GGTATCA246350.0106.6125958
GTGGTAT245700.0106.097796
CAGTGGT246300.0105.9865344
TGGTATC247100.0105.584717
GCAGTGG248950.0104.8292163
GGGTATC1450.075.0128867
GGGGTAT1650.065.920416
AGGGGTA1850.058.793885
AAATATA10050.055.546707145
ACGCACC752.5458727E-548.332848145
AAACGAC450.00963214648.332848145
ACGATCA450.00963214648.332848145
AGTACCC657.3276385E-444.614933145
GGACGAT700.001055356341.428158145
GTGTTAG907.4687196E-540.277374145
Produced by FastQC (version 0.11.5)