FastQCFastQC Report
Sun 19 Mar 2023
SRR4896138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161715
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGAGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT2670.16510527780354328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA100.007099204144.905659
AGCGTAC151.2328199E-4144.860866
GCGAATG700.0144.860867
GCTATAG500.0144.860867
CGAGAAT351.0913936E-11144.860868
GCGGAAT550.0144.860867
CGGTATG306.511982E-10144.860868
CAGTAGT151.2328199E-4144.860868
AGCTAGC351.0913936E-11144.860866
CGGTAAG151.2328199E-4144.860868
GCTAAGG450.0144.860867
GCGTCAG151.2328199E-4144.860867
GCGTATA500.0144.860867
CTAGCTG151.2328199E-4144.860868
CGTGCGA100.007105773144.860848
CATGATG100.007105773144.860848
CGATCGA100.007105773144.860848
GCATACT202.1353317E-6144.860847
AAGGAGC162250.0144.36982
AGGAGCT55950.0144.343033
Produced by FastQC (version 0.11.5)