FastQCFastQC Report
Sun 19 Mar 2023
SRR4896138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161715
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG98626.098382957672448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17771.098846736542683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13560.8385121973842872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4800.29681847695019015No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2730.16881550876542065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2610.1613950468416659No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCTGTCTCTA2390.14779086664811553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGATGGGTGACCACCT2190.13542343010852426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGATTCCCAAAAAGAATG2150.132949942800606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA1900.11749064712611693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA1710.10574158241350524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT145650.0104.8564151
AGCAGTG149300.0102.358492
AGTGGTA150250.0101.213985
GTATCAA150700.0101.169199
CAGTGGT151100.0100.850994
GGTATCA151850.0100.62518
GTGGTAT151700.0100.2465446
TGGTATC152350.099.866457
GCAGTGG153650.099.1772463
GGTGCGC554.372123E-879.088036145
GGGGTAT755.4569682E-1177.3544546
AGGGGTA902.7830538E-1064.462045
TGCACAT451.1910587E-464.442116145
TAGGGTG350.003561611862.140602145
ATTTCCG350.003561611862.140602145
TTAGCCC606.781369E-660.414474145
CGAATCT400.0060426254.373028145
CAGGGGT1203.5597623E-948.3764534
GGGTATC1203.5797711E-948.346537
AAGTAAC450.00962601848.331585145
Produced by FastQC (version 0.11.5)