FastQCFastQC Report
Sun 19 Mar 2023
SRR4896137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317568
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG23320.7343309149536477No Hit
ATTGGCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC20100.6329353083434099No Hit
ATTGGCTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA5430.17098700120918986No Hit
ATTGGCTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT4710.1483146916565901No Hit
ATTGGCTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATACC3910.12312323659814592No Hit
ATTGGCTCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3250.10234028617492946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT318050.0144.688891
TTGGCTC319100.0144.212782
TGGCTCA94250.0144.071043
GCTCATG16000.0144.010975
TGGCTCG100000.0143.757373
GGCTCGA28200.0143.631974
GGCTCAT37850.0143.385224
CTCGATG4200.0143.191516
GCTCGAG8100.0143.127625
TGGCTCT112350.0142.401473
GCTCAAT10050.0142.032795
GGCTCTG46800.0141.975024
CTCAGTA2300.0141.766346
GGCTCGT36550.0141.546554
GCTCGAT10250.0141.382165
GGCTCAA27900.0141.28084
GCTCTGA9700.0141.181735
GGCTCAG28900.0141.15594
GCTCATA9400.0141.062555
GCTCTAG9350.0140.266975
Produced by FastQC (version 0.11.5)