FastQCFastQC Report
Sun 19 Mar 2023
SRR4896136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626399
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC163072.603292789420162No Hit
CATCAAGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATACCACTGC7440.11877413597403573No Hit
CATCAAGTTGGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC7380.11781628003876123No Hit
CATCAAGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATACCACTGC6720.10727986475074194No Hit
CATCAAGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATACCACTGC6500.10376772632140216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTA500.0144.896946
ATCAAGT626900.0144.769822
CATCAAG627000.0144.746731
TCAAGTG224050.0144.476583
TCAAGTT215950.0143.991123
CAAGTTG110850.0143.72054
TCAAGTA165400.0143.363883
CAAGTGG83250.0143.156434
CAAGTAG65700.0142.801774
CAAGTGA61350.0142.653234
CAAGTGT79100.0142.607184
TCAAGTC25150.0142.304353
CAAGTAT54800.0141.459614
CAAGTTA50250.0139.994964
AAGTTGA51650.0138.865425
AAGTTAG19950.0138.360245
CAAGTTT56300.0138.33414
CAAGTAA46100.0138.139274
AGTTGAT38250.0137.131236
CAAGTCG9300.0137.106784
Produced by FastQC (version 0.11.5)