FastQCFastQC Report
Sun 19 Mar 2023
SRR4896136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626399
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11164917.823942886243433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA134972.1546969263999465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28510.4551412119112579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27730.4426890847526896No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA24890.39735057048303074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG16280.2598982437711427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14730.23515363210988524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTTGA14060.22445757416598686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTGACGTGGCCAAAAAA13540.21615615606027466No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12160.19412546954896162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAATCCTGACGTGGCCAAAAAA10110.16139872509375017No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG9890.15788658666441038No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9360.14942552590281913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG8490.13553661484133914No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8000.1277141247032642No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7900.12611769814447343No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT7850.12531948486507802No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG7530.12021091987694744No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA7410.11829520800639849No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7280.11621985347997045No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7230.11542164020057503No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG6930.11063236052420262No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA6700.10696057943898377No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6660.10632200881546747No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6620.10568343819195114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAG6370.10169237179497412No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG6310.10073451585969964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA12100.053.320984145
AAGCAGT763750.045.823571
GTATCAA768750.045.6125649
GGTATCA769150.045.5903328
AGCAGTG770450.045.3664472
AGTGGTA771850.045.1678165
GTGGTAT773450.045.0743836
TGGTATC777000.044.91517
CAGTGGT778950.044.815554
GCAGTGG780050.044.7337573
GGGGTAT3450.035.7295346
AAATATA15950.032.26942145
AGGGGTA3950.031.206815
CATCGGA1306.484106E-427.88176145
CCTGGGA18100.026.433758145
GGGTATC4700.026.2277
AAACCGC7100.023.68771785-89
TACGCAC1550.023.3865750-54
CGCTGTT1550.001808815523.3847145
TGTGGGA5900.023.345066145
Produced by FastQC (version 0.11.5)