FastQCFastQC Report
Sun 19 Mar 2023
SRR4896135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837720
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG163681.9538748030368143No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC56070.6693167168027503No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGC29860.3564436804660268No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG22980.27431600057298383No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT19260.22990975504941982No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG17570.20973594995941364No Hit
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14100.16831399512963757No Hit
ATCATTCCATGTACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10490.1252208375113403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC839800.0144.555131
CATTCCG236500.0144.519213
TCATTCC841050.0144.340292
ATTCCGA67900.0143.957094
CATTCCA277700.0143.769443
ATTCCGT85000.0143.383094
ATTCCTG93150.0142.661684
ATTCCGG82600.0142.636734
CATTCCT283550.0142.592093
ATTCCAG76700.0141.988944
ATTCCAT134850.0141.156224
ATTCCTA91000.0141.015884
TTCCGAT25550.0139.245595
TTCCGTA25500.0138.666175
TTCCGGA20550.0136.807795
TTCCGAG20900.0136.596885
TTCCTAG29050.0136.187555
TCCGTAA7800.0135.627936
TTCCTGA24250.0134.758355
TCCGGTA8450.0134.627526
Produced by FastQC (version 0.11.5)