FastQCFastQC Report
Sun 19 Mar 2023
SRR4896135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837720
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG747338.920999856754047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA107301.2808575657737669No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58320.6961753330468414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG42080.5023158095783794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25700.3067850833213962No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA17060.20364799694408633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGGCCTTTCCAAGATTGC12910.1541087714272072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATTGCTGTTTTTGTTTT12480.14897579143389197No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8500.10146588358878861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT794500.090.2845841
AGTGGTA815900.087.8224265
GTATCAA823550.087.72339
GGTATCA824050.087.631328
AGCAGTG818950.087.5307542
TGGTATC822500.087.161787
GTGGTAT823300.086.9978266
CAGTGGT826100.086.7556154
GCAGTGG833450.085.9122543
GGGTATC5550.056.1731837
AGGGGTA5700.054.6949425
GGGGTAT6100.051.1083876
AGGTATC3850.039.546877
CGGTATC2900.037.5013437
GCGGTAT3050.035.6570136
GAGGTAT4350.035.0012556
CAGGGGT9500.032.8169674
CATCGGA1352.142139E-532.22145145
AGCGGTA3450.031.522875
AGAGGTA4900.031.072545
Produced by FastQC (version 0.11.5)