FastQCFastQC Report
Sun 19 Mar 2023
SRR4896134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775307
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA775950.0144.783871
TAGCGAC776250.0144.727942
AGCGACT272100.0144.346083
GCGACTA85300.0144.310684
AGCGACA228300.0143.826253
GCGACGT91200.0143.713614
AGCGACG253800.0143.677753
GCGACTG84850.0143.624444
GCGACTT100250.0143.604374
GCGACAT85650.0143.55184
CGACTAG25850.0143.503865
CGACGAT27200.0142.77435
GCGACAG73900.0142.356194
CGACTTA27500.0142.270635
GCGACGA76200.0142.05284
CGACGTT29650.0141.728595
CGACTGA22700.0141.713525
GACTGAT8950.0141.667166
CGACATG27900.0141.529335
CGACTAT31450.0141.449655
Produced by FastQC (version 0.11.5)