FastQCFastQC Report
Sun 19 Mar 2023
SRR4896134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775307
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG151031.9480025331900783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG55950.7216496175063556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49290.6357481616959476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32050.41338463344197846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA22510.2903366021459886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT221800.0102.898461
GGTATCA226050.0101.329718
GTGGTAT225800.0100.510656
CAGTGGT227150.0100.0409854
TGGTATC227150.0100.009067
AGCAGTG227900.099.998112
AGTGGTA227250.099.901245
GTATCAA230300.099.597919
GCAGTGG230350.098.8400963
ATAGCGA1900.057.2365381
CGATAAG1301.8189894E-1255.765335145
TAGCGAC1850.054.8716472
CGGTCTG2150.047.206005145
AAATATA22900.035.139465145
TTCGATA2302.7284841E-1134.67149145
AGCGGTA1751.0075928E-733.1469575
AGGTATC3150.032.2262087
CGGTATC1851.6410377E-731.3552277
GCGGTAT1902.0733205E-730.530096
GAGGTAT3350.030.3022546
Produced by FastQC (version 0.11.5)