FastQCFastQC Report
Sun 19 Mar 2023
SRR4896133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667591
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG206900.0144.768543
GGCTAAC668550.0144.691862
AGGCTAA668600.0144.681061
GCTAACT244650.0144.671693
CTAACTG87750.0144.248084
GCTAACA198200.0143.994723
CTAACGA60650.0143.833454
CTAACGT73850.0143.82944
CTAACGG70550.0143.778954
TAACGGT22550.0143.30215
CTAACAG68750.0142.48474
CTAACTA68700.0142.377474
TAACGAT22650.0142.349535
TAACGTG25150.0141.163455
CTAACTT88900.0141.159584
CTAACAT71100.0140.934334
TAACATG20900.0140.748575
ACGATTG3400.0140.646597
TAACTGT31550.0140.085985
TAACTAG22350.0140.045915
Produced by FastQC (version 0.11.5)