FastQCFastQC Report
Sun 19 Mar 2023
SRR4896133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667591
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG269354.034655949525982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86521.2960030917133394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA37290.5585755350206939No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA34950.5235241337885023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21450.32130451129508936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG20200.3025804721753289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT17050.25539589359353254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT11780.1764553446646225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAATACAGGACTCTTTCG11130.16671884432234707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAGATTTATAGGTAGAG11040.1653707135057243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG10260.1536869130949938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA9030.13526245860114952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA7890.11818613492392799No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7810.1169877964202633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC7650.11459111941293397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7050.10560358063544896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT571750.0114.4951
AGTGGTA584900.0111.449835
AGCAGTG586700.0111.416852
GGTATCA588750.0111.164368
GTATCAA589550.0111.0297859
CAGTGGT588600.0110.773884
GTGGTAT588800.0110.576186
TGGTATC591450.0110.178817
GCAGTGG596050.0109.450153
TTGCACG300.001935768972.49708145
AGGGGTA2900.062.5021635
GAGGTAT3100.051.4533966
CCGACTA450.00963927648.331387145
CCCGCAT604.9448747E-448.331383145
GGGTATC3800.047.699027
AGGTATC3400.046.9133877
CAGGGGT4050.044.7546354
GGGGTAT4050.044.7546356
AGAGGTA3900.042.757895
AGCGGTA1102.4347907E-432.9556855
Produced by FastQC (version 0.11.5)