FastQCFastQC Report
Sun 19 Mar 2023
SRR4896132_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896132_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637824
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT13000.20381798113586194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGC638500.0144.727552
AGCCATG638750.0144.670911
CATGCGA61700.0144.299354
CCATGCG218000.0144.188573
CCATGCA189700.0143.893513
CCATGCT209950.0143.816763
CATGCGG75250.0143.538624
CATGCGT79350.0143.516974
CATGCAG61700.0143.477464
CATGCTA59550.0143.426594
ATGCGTA22200.0143.25485
CATGCTG73600.0143.016274
CATGCAT66950.0142.28954
ATGCGAT21850.0141.902485
CATGCAA60850.0141.552954
ATGCGGT23700.0141.21845
CATGCTT76600.0141.00894
ATGCGGA20300.0140.96095
ATGCTGG24850.0140.805115
ATGCGAG18700.0140.625055
Produced by FastQC (version 0.11.5)