FastQCFastQC Report
Sun 19 Mar 2023
SRR4896132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637824
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG149682.3467288781858318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG58740.9209437086092715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31070.4871249749147101No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30620.48006973710616097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20140.3157610876981738No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9420.1476896447922938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC7360.11539233393538029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT184750.094.4363861
GGTATCA186700.093.690438
GTATCAA187150.093.08729
GTGGTAT186650.093.016156
AGTGGTA188600.092.054435
AGCAGTG189700.091.917352
TGGTATC189050.091.912037
CAGTGGT190650.091.116934
GCAGTGG195900.088.675053
CGGTCGA350.00356630162.141144145
AAATATA19550.041.904213145
CGTACTT907.480605E-540.276665145
GCCCCAA5950.040.208977145
TGCGGCT4750.030.525475145
GAGGTAT2202.255183E-829.6675786
ACTAGCG200.00607631329.0001170-74
AGGGGTA2456.412847E-826.6402725
AGAGGTA2756.7338988E-926.371185
AAACCGC11200.026.15106485-89
AGGTATC2351.3258687E-624.6879147
Produced by FastQC (version 0.11.5)