FastQCFastQC Report
Sun 19 Mar 2023
SRR4896131_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896131_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790922
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG24470.30938575485319664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT793050.0144.522921
GCACCTC794650.0144.231922
ACCTCGA78000.0143.336584
CACCTCG263750.0143.212523
ACCTCGT93950.0142.833444
ACCTCAG84850.0142.439334
CACCTCA251850.0142.326483
ACCTCAT94500.0142.002144
CTCGAAT8050.0141.31546
ACCTCGG89550.0141.112854
ACCTCTA79200.0140.52444
CACCTCT263000.0140.39753
ACCTCTG89650.0139.985584
CCTCATG37000.0139.628335
CCTCGTA28250.0139.273065
CTCGTAG8850.0139.184666
CTCAGTA8050.0138.61516
CCTCAGT26450.0138.067215
ACCTCAA72850.0137.854024
CCTCTAG24800.0137.611575
Produced by FastQC (version 0.11.5)